Fluoro nissl green: a novel fluorescent counterstain for neuroanatomy

Neurosci Lett. 1995 Jan 30;184(3):169-72. doi: 10.1016/0304-3940(94)11198-r.


Fluorescent neuroanatomic techniques, such as immunofluorescence and retrograde and anterograde tracing studies, derive great utility from their specificity. However, the specificity can be a drawback as well, in that it may be difficult to assess labeled neurons or neural processes in their cytoarchitectonic context. We report the characteristics of a newly synthesized fluorescent counterstain, Fluoro Nissl Green (3,8-diamino-10H-quindoline) with spectral characteristics similar to fluorescein. This Nissl-like counterstain can be used as a green neuronal counterstain for red-emitting markers such as rhodamine and Di-I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids*
  • Animals
  • Fluorescent Dyes*
  • Indoles*
  • Male
  • Neurons / cytology*
  • Quinolines*
  • Rats


  • 3,8-diamino-10H-quindoline
  • Alkaloids
  • Fluorescent Dyes
  • Indoles
  • Quinolines