Enhancement of anti-cancer activity of cisdiaminedichloroplatinum by the protein-bound polysaccharide of Coriolus versicolor QUEL (PS-K) in vitro

Cancer Biother. Winter 1994;9(4):351-8. doi: 10.1089/cbr.1994.9.351.

Abstract

The protein-bound polysaccharide of Coriolus versicolor QUEL (PS_K) expresses superoxide dismutase (SOD) mimicking activity. Examination was made of the effects of PS-K on cancer cell lines following administration of the anti-cancer drug cisdiaminedichloroplatinum (cisplatin). Cell proliferation of each cell line was inhibited markedly by cisplatin from 0.5 to 5 micrograms/0.5 ml per well. Fifty percent of the inhibitory concentration (IC50) was 0.33 micrograms/0.5 ml per well in NRK-49F and human ovarian cancer cells, and 1.5 micrograms/0.5 ml per well in H4-II-E. PS-K 50 micrograms/0.5 ml per well prevented cytotoxicity due to cisplatin toward NRK-49F, but enhanced the cytotoxicity on H4-II-E and human ovarian cancer cells. Increase in lipid peroxide and decrease in SOD activity were observed following an IC50 dose of cisplatin. With PS-K 50 micrograms/0.5 ml per well, all the above were augmented in H4-II-E and ovarian cancer cells, but diminished in NRK-49F cell line. PS-K may have effect on cancer patients through its combining with cisplatin.

MeSH terms

  • Animals
  • Antibiotics, Antineoplastic / pharmacology*
  • Catalase / metabolism
  • Cisplatin / pharmacology*
  • Glutathione Peroxidase / metabolism
  • Humans
  • Lipid Peroxides / metabolism
  • Proteoglycans / pharmacology*
  • Rats
  • Superoxide Dismutase / metabolism
  • Tumor Cells, Cultured

Substances

  • Antibiotics, Antineoplastic
  • Lipid Peroxides
  • Proteoglycans
  • polysaccharide-K
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Cisplatin