Identification and Purification of Human Stat Proteins Activated in Response to interleukin-2

Immunity. 1995 Apr;2(4):321-9. doi: 10.1016/1074-7613(95)90140-x.

Abstract

A key cytokine induced during the immune response is IL-2. Following T cell activation, the genes encoding IL-2 and the various chains of its receptor are transcriptionally induced. In turn, secreted IL-2 serves to stimulate the proliferation and differentiation of T lymphocytes. Several recent studies have implicated Jak kinases in the signaling pathway induced by IL-2. Following this lead, we set out to identify transcription factors induced in response to IL-2. Human peripheral blood lymphocytes were observed to contain several IL-2-inducible DNA binding activities. Similar activities were also observed in a transformed human lymphocyte line, termed YT. We have purified these activities and found that the principal IL-2-inducible component bears significant relatedness to a prolactin-induced transcription factor first identified in sheep mammary gland tissue. We hypothesize that activation of this protein, designated hStat5, helps govern the biological effects of IL-2 during the immune response.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Line, Transformed
  • Cloning, Molecular
  • DNA, Complementary
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / isolation & purification
  • Humans
  • Interleukin-2 / pharmacology*
  • Lymphocyte Activation
  • Lymphocytes / immunology
  • Lymphocytes / metabolism*
  • Milk Proteins*
  • Molecular Sequence Data
  • STAT5 Transcription Factor
  • Sequence Alignment
  • Trans-Activators / genetics*
  • Trans-Activators / isolation & purification
  • Transcription Factors / genetics
  • Transcription Factors / isolation & purification
  • Tumor Suppressor Proteins

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • Interleukin-2
  • Milk Proteins
  • STAT5 Transcription Factor
  • STAT5A protein, human
  • Trans-Activators
  • Transcription Factors
  • Tumor Suppressor Proteins

Associated data

  • GENBANK/L41142