Production of interleukin-6, tumor necrosis factor alpha and interleukin-10 in vitro correlates with the clinical immune defect in chronic hemodialysis patients

Kidney Int. 1995 Feb;47(2):559-65. doi: 10.1038/ki.1995.70.


In patients with chronic renal failure alterations in monokine production are a common feature. Their clinical relevance has not yet been proven. We show here a correlation between an overproduction of interleukin-(IL)-6 and tumor necrosis factor alpha (TNF alpha) upon stimulation with LPS by mononuclear cells in vitro and the clinical grade of immunodeficiency found in these patients. Higher levels of IL-6 and TNF alpha were correlated with an immunocompromized state, that is, non-responsiveness to hepatitis B vaccination, whereas patients with a better immune competence showed the same levels of these cytokines as healthy controls. Only the patients with a good immune function showed a high secretion of IL-10. The feedback mechanism of IL-10 for reducing monokine synthesis seems to be intact in these patients. Thus the secretion of IL-10 might be regarded as a compensatory mechanism which controls monokine induction by chronic renal failure and hemodialysis treatment. Immunocompromized patients who are unresponsive to hepatitis B vaccination seem to be unable to enhance IL-10 synthesis for control of monokine overproduction. This results in higher levels of IL-6 and TNF alpha that might be involved in the pathogenesis of reduced immune defense.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Base Sequence
  • Hepatitis B / prevention & control
  • Humans
  • Immune System Diseases / metabolism*
  • Interleukin-10 / biosynthesis*
  • Interleukin-10 / immunology
  • Interleukin-10 / pharmacology
  • Interleukin-6 / biosynthesis*
  • Interleukin-6 / blood
  • Lipopolysaccharides / pharmacology
  • Molecular Sequence Data
  • Monokines / metabolism
  • Oligonucleotide Probes / genetics
  • Recombinant Proteins
  • Renal Dialysis*
  • Time Factors
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Vaccination


  • Antibodies, Monoclonal
  • Interleukin-6
  • Lipopolysaccharides
  • Monokines
  • Oligonucleotide Probes
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Interleukin-10