Exposure of neuronal cultures to K+ depolarization or to N-methyl-D-aspartate increases the transcription of genes encoding the alpha 1 and alpha 5 GABAA receptor subunits

Brain Res Mol Brain Res. 1995 Feb;28(2):338-42. doi: 10.1016/0169-328x(94)00240-f.

Abstract

The transcription rates of the alpha 1, alpha 5, and alpha 6 gamma-aminobutyric acidA receptor subunit genes were analyzed in cultures maintained in low KCl (12.5 mM), in low KCl treated with NMDA (10 microM), and in high KCl (25 mM). alpha 1 and alpha 5 transcription rates were significantly increased in response to NMDA or high KCl treatment, while alpha 6 and cyclophilin transcription rates were not changed by either condition. These data suggest that following NMDA or high KCl treatment of granule cells, changes in alpha 1 and alpha 5 mRNA content are a consequence of a specific transcriptional rate increase of the corresponding subunit genes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Cells, Cultured
  • Cerebellum / physiology*
  • Gene Expression
  • N-Methylaspartate / pharmacology*
  • Neurons / physiology*
  • Potassium / pharmacology*
  • Rats
  • Receptors, GABA-A / genetics*
  • Receptors, GABA-A / physiology

Substances

  • Receptors, GABA-A
  • N-Methylaspartate
  • Potassium