Cloning and expression of a bovine glutamate transporter

Brain Res Mol Brain Res. 1995 Feb;28(2):343-8. doi: 10.1016/0169-328x(94)00244-9.


We have isolated a 3845 base-pair cDNA (BNGLUAS) encoding a bovine glutamate transporter (bovine GLAST) by screening a bovine retina cDNA library with an oligonucleotide probe corresponding to conserved regions of known glutamate transporters. The cDNA sequence predicted a protein of 542 amino acids and displayed 96% and 97% amino acid identity with the rat GLAST/GluT-1 and human GLAST, respectively. Expression of the bovine GLAST in Xenopus oocytes revealed Na(+)-dependent [14C]L-glutamate uptake and electrogenic glutamate uptake.

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • Amino Acid Transport System X-AG
  • Animals
  • Base Sequence
  • Cattle
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression / genetics*
  • Glutamic Acid / metabolism
  • Humans
  • Mice
  • Molecular Sequence Data
  • Oocytes
  • Rats
  • Retina / physiology
  • Sequence Analysis
  • Sodium / pharmacology
  • Xenopus


  • ATP-Binding Cassette Transporters
  • Amino Acid Transport System X-AG
  • DNA, Complementary
  • Glutamic Acid
  • Sodium

Associated data

  • GENBANK/D82056