Isolation of transducing phage carrying rps T, the structural gene for ribosomal protein S20

Mol Gen Genet. 1976 Feb 27;144(1):115-8. doi: 10.1007/BF00277314.

Abstract

Lambda transducing phages carrying segments of the Escherichia coli chromosome in the dapB region have been isolated in their in vivo gene products analyzed by two-dimensional gel electrophoresis. One of these phages, lambdaddapB-2, carries the structural genes for ribosomal protein S20 (rps T) and isoleucyltransfer RNA synthetase (ileS.) The most likely gene order is thr-rpsT-ileS-dapB-pyrA.

MeSH terms

  • Bacterial Proteins / biosynthesis*
  • Chromosome Mapping
  • Chromosomes, Bacterial
  • Coliphages / isolation & purification*
  • Coliphages / metabolism
  • DNA, Bacterial
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / metabolism*
  • Genes*
  • Ribosomal Proteins / biosynthesis*
  • Transduction, Genetic

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Ribosomal Proteins