Recombination and hydroxyurea inhibition of DNA synthesis in yeast meiosis

Mol Gen Genet. 1976 Feb 27;144(1):21-7. doi: 10.1007/BF00277299.


Hydroxyurea (HU) inhibits the premeiotic DNA replication and the meiotic events that followed, namely readiness, recombination commitment, haploidisation, sporulation commitment and ascus formation. Short incubations with HU (2-4 hrs) during the premeiotic replication (i.e. starting between 3 and 6.5 hrs in sporulation medium) allow the resumption of the replication at a normal rate following the removal of the drug. The other meiotic events are similarly delayed by the approximate length of the treatment. In these experiments, intragenic recombination in ade2 reached a higher level than in the controls (x 1.3-2.0 in one pair of heteroalleles and x 3.0-4.0 in another pair). The recombination response to short HU treatments was not observed for a pair of heteroalleles in ade2 that normally shows a high level of meiotic recombination (750 per 10(6) cells), nor was the response observed in a pair of heteroalleles in lys2. HU treatments have almost no effect on sporulating cells from 8 hrs onwards. At 7-7.5 hrs the meiotic cells are very sensitive to the drug and even short treatments cause cell death and massive DNA degradation.

MeSH terms

  • DNA Replication / drug effects*
  • Diploidy
  • Gene Frequency
  • Hydroxyurea / pharmacology*
  • Meiosis*
  • Recombination, Genetic*
  • Saccharomyces cerevisiae / metabolism*
  • Spores, Fungal
  • Time Factors


  • Hydroxyurea