Benomyl affects the microtubule cytoskeleton and the glutathione level of mammalian primary cultured hepatocytes

Toxicol Lett. 1995 Mar;76(2):135-44. doi: 10.1016/0378-4274(94)03206-m.


Rat primary hepatocyte cultures have been used to study the effect of Benomyl alone or in combination with Pirimiphos-methyl. The results presented demonstrate that Benomyl alone is responsible for the microtubular disorganization in both a time- and dose-dependent manner, that the effect is reversible after the agent is removed, and that Benomyl is a potent glutathione-depleting agent. Pirimiphos-methyl, alone or combined with Benomyl had no effect on microtubule organization, but reinforced the decrease in glutathione.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Benomyl / toxicity*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Cytoskeleton / drug effects*
  • Dose-Response Relationship, Drug
  • Fluorescent Antibody Technique
  • Glutathione / drug effects
  • Glutathione / metabolism*
  • Liver / cytology
  • Liver / drug effects
  • Male
  • Microtubules / drug effects*
  • Rats
  • Rats, Sprague-Dawley
  • Sulfhydryl Compounds / physiology*
  • Time Factors


  • Antibodies, Monoclonal
  • Sulfhydryl Compounds
  • Glutathione
  • Benomyl