Binding of pesticides to alpha, mu and pi class glutathione transferase

Toxicol Lett. 1995 Mar;76(2):173-7. doi: 10.1016/0378-4274(94)03210-x.


The binding of fluorodifen, fenarimol, acifluorfen, 2,4-DES, methyl parathion, paraquat and pyrazophos by alpha, mu and pi class glutathione transferases (GST) was determined by the 2-p-toluidinylnaphthalene-6-sulphonate (TNS) binding fluorescence inhibition technique. Although all the 3 GST classes appear to be capable of binding the pesticides investigated, mu class exhibited somewhat higher affinity than the alpha and pi classes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Fluorescent Dyes
  • Glutathione Transferase / metabolism*
  • Humans
  • In Vitro Techniques
  • Mice
  • Naphthalenesulfonates
  • Pesticides / metabolism*
  • Protein Binding
  • Spectrometry, Fluorescence


  • Fluorescent Dyes
  • Naphthalenesulfonates
  • Pesticides
  • 2-(4-toluidino)-6-naphthalenesulfonic acid
  • Glutathione Transferase