Development of a gas chromatography-mass spectrometry method for determination of ketamine in plasma and its application to human samples

Ther Drug Monit. 1995 Feb;17(1):95-100. doi: 10.1097/00007691-199502000-00016.


A sensitive and precise gas chromatography-mass spectrometry method with selected ion monitoring has been developed for identification and quantification of the phencyclidine derivative ketamine in human plasma. The assay is based on an alkaline extraction from aqueous to organic solvent from plasma and an efficient gas chromatographic separation on a DB-5 capillary column. The analytical procedure has a coefficient of variation of 0.7-6.2% and from 1.3 to 8.7% within-day and from day-to-day analysis, respectively. The low level of sensitivity was 10 ng/ml. It was used to measure low plasma concentrations in volunteers during ketamine-induced experimental psychosis. The method is not enantio selective.

Publication types

  • Clinical Trial

MeSH terms

  • Adult
  • Chromatography, High Pressure Liquid
  • Female
  • Gas Chromatography-Mass Spectrometry / methods
  • Humans
  • Ketamine / blood*
  • Ketamine / pharmacokinetics
  • Male
  • Psychoses, Substance-Induced / blood


  • Ketamine