Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro

Proc Natl Acad Sci U S A. 1976 Apr;73(4):1053-7. doi: 10.1073/pnas.73.4.1053.


E. coli dnaZ gene product is required for conversion of phiX174, fd, and ST-1 single-stranded phage DNAs to duplex DNAs in vitro. This protein has been purified about 5000-fold. It functions in the elongation of RNA- or DNA-primed single-stranded DNA that is catalyzed by DNA polymerase III(DNA nucleotidyltransferase; deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase; EC in conjunctions with two other E. coli protein preparations referred to as DNA elongation factors I and III. It also functions in similar reactions catalyzed by DNA polymerase II in combination with E. coli DNA binding protein and DNA elongation factors I and III.

MeSH terms

  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism*
  • Cell-Free System
  • DNA Nucleotidyltransferases / metabolism
  • DNA Replication
  • DNA, Bacterial / biosynthesis*
  • DNA, Single-Stranded / metabolism
  • Escherichia coli / metabolism*
  • Genes*


  • Bacterial Proteins
  • DNA, Bacterial
  • DNA, Single-Stranded
  • DNA Nucleotidyltransferases