Signals from the IL-1 receptor homolog, Toll, can activate an immune response in a Drosophila hemocyte cell line

Biochem Biophys Res Commun. 1995 Apr 6;209(1):111-6. doi: 10.1006/bbrc.1995.1477.


The Toll gene encodes an interleukin 1 receptor-like protein that mediates dorsoventral polarity in the Drosophila embryo. The possible involvement of Toll or Toll-like proteins also in the Drosophila immune response was investigated by overexpressing Toll10B, a constitutively active mutant protein, in the Drosophila blood cell line mbn-2. Induction of the Cecropin A1 (CecA1) gene, coding for a bactericidal peptide, was used as an indicator for the immune response. Toll10B was found to increase CecA1 transcription, as detected with a cotransfected CecA1-lacZ reporter gene construct. This effect depends on the presence of a kappa B-like site in the CecA1 promoter. The endogenous Toll gene is expressed in mbn-2 cells, indicating that this gene may normally play a role in Drosophila blood cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / metabolism
  • Antimicrobial Cationic Peptides*
  • Cell Line
  • Drosophila
  • Drosophila Proteins*
  • Gene Expression Regulation / drug effects
  • Hemocytes / immunology*
  • Insect Hormones / genetics
  • Insect Hormones / metabolism*
  • Lipopolysaccharides / pharmacology
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism*
  • NF-kappa B / metabolism
  • Promoter Regions, Genetic
  • Receptors, Cell Surface*
  • Receptors, Interleukin-1 / metabolism*
  • Signal Transduction*
  • Toll-Like Receptors


  • Anti-Bacterial Agents
  • Antimicrobial Cationic Peptides
  • Drosophila Proteins
  • Insect Hormones
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • NF-kappa B
  • Receptors, Cell Surface
  • Receptors, Interleukin-1
  • Tl protein, Drosophila
  • Toll-Like Receptors
  • cecropin A