cDNA cloning of murine Nrf 2 gene, coding for a p45 NF-E2 related transcription factor

Biochem Biophys Res Commun. 1995 Apr 6;209(1):40-6. doi: 10.1006/bbrc.1995.1467.

Abstract

A lambda gt11 cDNA library was constructed from poly-A rich RNA prepared from circulating murine yolk sac derived nucleated erythroblasts, on day 9 of gestation. In addition to the embryonic globin genes, this library was found to contain clones for the hematopoietic specific p45 NF-E2, as well as p18 NF-E2 and p45 NF-E2 related factor 1 (Nrf 1) genes. Using a degenerate oligonucleotide 17mer probe coding for a part of the highly conserved DNA binding domain for p45 NF-E2, we have isolated murine Nrf 2, a second murine homologue related to p45 NF-E2. The murine Nrf 2 gene is expressed not only in erythroid cells, but also in the 3T3 murine fibroblast cell line.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary
  • DNA-Binding Proteins / genetics*
  • Erythrocytes / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • NF-E2-Related Factor 2
  • Oligonucleotide Probes
  • Sequence Homology, Amino Acid
  • Trans-Activators / genetics*
  • Yolk Sac / cytology

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • NF-E2-Related Factor 2
  • Nfe2l2 protein, mouse
  • Oligonucleotide Probes
  • Trans-Activators

Associated data

  • GENBANK/U20532