No involvement of Ki-ras or p53 gene mutations in colitis-associated rat colon tumors induced by 1-hydroxyanthraquinone and methylazoxymethanol acetate

Mol Carcinog. 1995 Apr;12(4):193-7. doi: 10.1002/mc.2940120403.

Abstract

1-Hydroxyanthraquinone (1-HA), which is present in some herbs, and methylazoxymethanol (MAM) acetate, a metabolite of azoxymethane, show synergistic carcinogenicity in rat colon, and 1-HA induces ulcerative changes with simultaneous severe inflammation of the entire colon. In this study, mutations in Ki-ras (exons 1 and 2) and p53 (exons 4-7) were studied by polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) analysis. Of 18 adenomas and 38 adenocarcinomas induced in male F344 rats (52 tumors induced by 1-HA plus MAM acetate, three by 1-HA alone, and one by MAM acetate alone), no mutations in Ki-ras or p53 were detected under two conditions of PCR-SSCP analysis. Because human colon carcinomas from patients with ulcerative colitis have a very low incidence of Ki-ras mutation, this experimental system would be a good animal model of human colon carcinomas with ulcerative colitis and of human colon carcinomas without Ki-ras or p53 mutations.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthraquinones
  • Base Sequence
  • Codon
  • Colitis / chemically induced
  • Colitis / complications
  • Colitis / genetics*
  • Colonic Neoplasms / chemically induced
  • Colonic Neoplasms / genetics*
  • Colonic Neoplasms / pathology
  • DNA Primers
  • Exons
  • Genes, p53*
  • Genes, ras*
  • Male
  • Methylazoxymethanol Acetate
  • Molecular Sequence Data
  • Mutagenesis*
  • Polymerase Chain Reaction
  • Rats
  • Rats, Inbred F344

Substances

  • Anthraquinones
  • Codon
  • DNA Primers
  • Methylazoxymethanol Acetate
  • 1-hydroxyanthraquinone