Cloning and characterization of hTAFII18, hTAFII20 and hTAFII28: three subunits of the human transcription factor TFIID

EMBO J. 1995 Apr 3;14(7):1520-31. doi: 10.1002/j.1460-2075.1995.tb07138.x.

Abstract

We have cloned cDNAs encoding three novel TAFIIs [TATA-binding protein (TBP)-associated factors] from the human (h) HeLa cell TFIID complexes hTAFII28, hTAFII20 and hTAFII18. hTAFII28 is a core hTAFII present in both of the previously described hTFIID species which either lack or contain hTAFII30 (hTFIID alpha and hTFIID beta respectively), and is the homologue of Drosophila (d)TAFII30 beta. hTAFII18 is a novel hTAFII which shows homology to the N-terminal region of the yeast TAFIISPT3, but has no known Drosophila counterpart. In contrast to hTAFII28, hTAFII18 is a TFIID beta-specific hTAFII. hTAFII20 is the homologue of p22, an alternatively spliced form of dTAFII30 alpha (p32). Using a combination of protein affinity chromatography and cotransfection and immunoprecipitation assays, we have identified a series of in vitro and intracellular interactions among the novel hTAFIIs and between the novel hTAFIIs and hTAFII30 or TBP. We show that hTAFII28 interacts with hTAFII18 both in vitro and intracellularly; in contrast to its Drosophila homologue, hTAFII28 also interacts directly with TBP. Deletion analysis indicates that TBP and hTAFII18 bind to distinct domains of hTAFII28. hTAFII18 also interacts with TBP, but it interacts more strongly with hTAFII28 and hTAFII30. The binding of hTAFII28 and hTAFII30 requires distinct domains of hTAFII18. As observed with the homologous Drosophila proteins, hTAFII20 interacts directly with TBP; however, additional interactions between hTAFII20 and hTAFII28 or hTAFII30 were detected. These results reveal differences not only in subunit composition, but also in the organization of dTFIID and hTFIID complexes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Chromatography, Affinity
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • DNA-Binding Proteins / metabolism
  • Drosophila / genetics
  • Drosophila / metabolism
  • Humans
  • Macromolecular Substances
  • Models, Structural
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Protein Biosynthesis
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • TATA Box
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors / biosynthesis*
  • Transcription Factors / chemistry
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • Transfection

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • Macromolecular Substances
  • Oligodeoxyribonucleotides
  • Recombinant Proteins
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors

Associated data

  • GENBANK/X83928
  • GENBANK/X84002
  • GENBANK/X84003