Plasmodium falciparum: detection of antifolate resistance by mutation-specific restriction enzyme digestion

Exp Parasitol. 1995 May;80(3):483-7. doi: 10.1006/expr.1995.1060.

Abstract

We describe here a rapid procedure to predict the resistance of Plasmodium falciparum to pyrimethamine or cycloguanil. The method consists of amplification by PCR of the DHFR gene followed by restriction enzyme digestion of codons 16 and 108. Three different enzymes are used to cut the wild-type, 108-threonine mutant, and 108-asparagine mutant gene. Since every natural antifolate-resistant isolate identified until now carries a mutation in codon 108, determination of the nature of this codon can predict the sensitivity of any P. falciparum isolate.

MeSH terms

  • Animals
  • Base Sequence
  • Codon / chemistry
  • Codon / genetics
  • DNA Primers / chemistry
  • Drug Resistance / genetics
  • Genes, Protozoan
  • Humans
  • Molecular Sequence Data
  • Plasmodium falciparum / drug effects*
  • Plasmodium falciparum / genetics
  • Point Mutation*
  • Polymerase Chain Reaction
  • Proguanil
  • Pyrimethamine / pharmacology*
  • Restriction Mapping
  • Tetrahydrofolate Dehydrogenase / genetics*
  • Triazines / pharmacology*

Substances

  • Codon
  • DNA Primers
  • Triazines
  • cycloguanil
  • Tetrahydrofolate Dehydrogenase
  • Proguanil
  • Pyrimethamine