A new member of the cytosolic O-acetylserine(thiol)lyase gene family in Arabidopsis thaliana

FEBS Lett. 1995 Apr 17;363(1-2):1-5. doi: 10.1016/0014-5793(95)00255-8.


A cDNA, Atcys-3A, encoding O-acetylserine-(thiol)lyase has been isolated from Arabidopsis thaliana. The deduced peptide sequence showed a high level of similarity with the bacterial counterpart, and a remarkable percentage of identity with other higher plant O-acetylserine(thiol)lyase genes. Sequence comparison and Southern blot analysis suggested that Atcys-3A was a new and different to the previously reported member of the cytosolic gene family in Arabidopsis. The Atcys-3A expression was activated by sulfur limitation, requiring a carbon and nitrogen source for maximal expression. A similar pattern of regulation was observed at the O-acetylserine(thiol)lyase activity level. Northern blot analysis also showed an organ-specific expression of Atcys-3A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Base Sequence
  • Blotting, Northern
  • Cysteine Synthase / chemistry
  • Cysteine Synthase / genetics*
  • Cytosol / enzymology*
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification
  • Gene Expression Regulation, Plant
  • Genes, Plant*
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Sequence Analysis, DNA


  • DNA, Complementary
  • RNA, Messenger
  • Cysteine Synthase

Associated data

  • GENBANK/X84097