Affinity purification and cDNA cloning of rat neural differentiation and tumor cell surface antigen gp130RB13-6 reveals relationship to human and murine PC-1

J Biol Chem. 1995 Apr 28;270(17):9849-55. doi: 10.1074/jbc.270.17.9849.


Monoclonal antibody RB13-6 recognizes a subset of rat brain glial precursor cells that are highly susceptible to malignant conversion by the carcinogen N-ethyl-N-nitrosourea. The corresponding cell surface antigen was identified as a membrane glycoprotein (gp130RB13-6) and purified by immunoaffinity chromatography from the tumorigenic neuroectodermal rat cell line BT4Ca. Sequencing of 5 endoproteinase-generated peptides of the purified antigen permitted the specific amplification of a cDNA fragment by reverse transcription-polymerase chain reaction and subsequent isolation of the complete coding sequence from a fetal rat brain cDNA library. The derived amino acid sequence indicates that the RB13-6 antigen is related to the human and murine plasma cell membrane protein PC-1, a nucleotide pyrophosphatase/alkaline phosphodiesterase and ectoprotein kinase. Similarly, purified gp130RB13-6 possesses 5'-nucleotidase activity that can be inhibited with EDTA. Different from PC-1, gp130RB13-6 isolated from BT4Ca cells is not a disulfide-linked dimer and contains an RGD-tripeptide sequence which, together with other structural features, suggests a possible function in cell adhesion and its subversion in malignant phenotypes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation, B-Lymphocyte / genetics*
  • Base Sequence
  • Cell Line
  • Chromatography, Affinity / methods
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary
  • Humans
  • Lysosomal Membrane Proteins
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / isolation & purification
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / isolation & purification
  • Rats
  • Sequence Homology, Amino Acid


  • Antigens, Differentiation, B-Lymphocyte
  • DNA Primers
  • DNA, Complementary
  • LAMP1 protein, human
  • Lamp1 protein, mouse
  • Lamp1 protein, rat
  • Lysosomal Membrane Proteins
  • Membrane Glycoproteins
  • Neoplasm Proteins

Associated data

  • GENBANK/Z47987