Ets-related protein E1A-F can activate three different matrix metalloproteinase gene promoters

Oncogene. 1995 Apr 6;10(7):1461-3.


An Ets-related E1A-F has been characterized as an enhancer-binding protein for the adenovirus E1A gene. Here we show, in transient expression assays, that E1A-F can activate three different subclasses of the matrix metalloproteinase gene promoters. Expressions of the chloramphenicol acetyltransferase (CAT) reporter gene under the control of stromelysin, type I collagenase and 92 kD type IV collagenase promoters were increased approximately 10- to 20-fold by co-transfection with the E1A-F expression vector. Activation levels were as much high as those obtained by exogenous expression of AP-1 transcription factor. These results suggest that E1A-F positively regulates transcriptions from matrix metalloproteinase genes that are associated with invasion and metastasis of tumor cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus E1A Proteins / physiology*
  • DNA-Binding Proteins / physiology*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • In Vitro Techniques
  • Matrix Metalloproteinase 3
  • Metalloendopeptidases / genetics*
  • Osteosarcoma
  • Promoter Regions, Genetic
  • Proto-Oncogene Proteins / physiology*
  • Proto-Oncogene Proteins c-ets
  • RNA, Messenger / genetics
  • Transcription Factors / physiology*
  • Transcription, Genetic
  • Tumor Cells, Cultured


  • Adenovirus E1A Proteins
  • DNA-Binding Proteins
  • ETV4 protein, human
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-ets
  • RNA, Messenger
  • Transcription Factors
  • Metalloendopeptidases
  • Matrix Metalloproteinase 3