Molecular cloning and functional characterization of the human platelet-derived growth factor alpha receptor gene promoter

Oncogene. 1995 Apr 20;10(8):1667-72.


Expression of the platelet-derived growth factor alpha receptor (PDGF alpha R) is strictly regulated during mammalian development and tumorigenesis. The molecular mechanisms involved in the specific regulation of PDGF alpha R expression are unknown, but transcriptional regulation of the PDGF alpha R gene is most likely to be involved. This study describes the molecular cloning of the non-coding exon 1 and approximately 2 kb of 5' flanking region of the human PDGF alpha R gene. This 5' flanking region is a functional promoter of the PDGF alpha R gene as concluded from its capacity to drive luciferase reporter gene expression in an orientation dependent way. Analysis of 5' promoter deletion mutants revealed that the region from -441 to +118, relative to the transcription initiation site, is sufficient to establish high level promoter activity. In addition, the morphogen retinoic acid, alone or in combination with dibutyryl cAMP, gives a 22-fold induction of PDGF alpha R gene promoter activity in human teratocarcinoma cells. This effect is mediated through specific transcription factor binding within the -52/+118 region of the PDGF alpha R gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Bucladesine / pharmacology
  • Cloning, Molecular
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Receptors, Platelet-Derived Growth Factor / genetics*
  • TATA Box
  • Theophylline / pharmacology
  • Tretinoin / pharmacology


  • Tretinoin
  • Bucladesine
  • Theophylline
  • Receptors, Platelet-Derived Growth Factor

Associated data

  • GENBANK/X80389