Oral immunization with a recombinant bacterial antigen produced in transgenic plants

Science. 1995 May 5;268(5211):714-6. doi: 10.1126/science.7732379.

Abstract

The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. The plants expressed the foreign peptides, both of which formed oligomers that bound the natural ligand. Mice immunized by gavage produced serum and gut mucosal anti-LT-B immunoglobulins that neutralized the enterotoxin in cell protection assays. Feeding mice fresh transgenic potato tubers also caused oral immunization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Administration, Oral
  • Amino Acid Sequence
  • Animals
  • Bacterial Toxins / immunology
  • Bacterial Vaccines / administration & dosage*
  • Bacterial Vaccines / biosynthesis*
  • Base Sequence
  • Enterotoxins / immunology
  • Escherichia coli / immunology
  • Escherichia coli Proteins*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Nicotiana
  • Plants, Genetically Modified / immunology*
  • Plants, Toxic
  • Protein Sorting Signals
  • Recombinant Fusion Proteins / immunology
  • Solanum tuberosum
  • Vaccines, Synthetic / administration & dosage*
  • Vaccines, Synthetic / biosynthesis*

Substances

  • Bacterial Toxins
  • Bacterial Vaccines
  • Enterotoxins
  • Escherichia coli Proteins
  • Protein Sorting Signals
  • Recombinant Fusion Proteins
  • Vaccines, Synthetic
  • SEKDEL sequence
  • heat-labile enterotoxin, E coli