Cloning and functional characterization of the human vasoactive intestinal peptide (VIP)-2 receptor

Biochem Biophys Res Commun. 1995 Apr 17;209(2):385-92. doi: 10.1006/bbrc.1995.1515.


A full-length clone encoding the human VIP-2 receptor was isolated from a human placenta cDNA library. The 1317-bp cDNA insert encodes a protein of 438 amino acids which is a member of the seven transmembrane domain G-protein-coupled receptor superfamily. Expression of the human VIP-2 receptor in COS-7 cells confered high affinity binding to [125I] VIP (IC50 = 0.93 nM), which was displaced by unlabeled PACAP-38 (IC50 = 6.2 nM). VIP and PACAP-38 were equipotent in stimulating accumulation of cAMP in COS-7 cells transfected with the human VIP-2 receptor. Northern blot analysis revealed two VIP-2 receptor mRNAs of 4.6 kb and 2.3 kb in size which were expressed predominantly in the human skeletal muscle and to a lesser extent in the human brain, heart, pancreas and placenta.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Gene Expression
  • Humans
  • Molecular Sequence Data
  • Oligonucleotide Probes / chemistry
  • RNA, Messenger / genetics
  • Rats
  • Receptors, Vasoactive Intestinal Peptide / genetics*
  • Receptors, Vasoactive Intestinal Peptide, Type II
  • Sequence Alignment
  • Sequence Homology, Amino Acid


  • Oligonucleotide Probes
  • RNA, Messenger
  • Receptors, Vasoactive Intestinal Peptide
  • Receptors, Vasoactive Intestinal Peptide, Type II
  • VIPR2 protein, human

Associated data

  • GENBANK/L40764