Mouse multidrug resistance 1a/3 gene is the earliest known endothelial cell differentiation marker during blood-brain barrier development

Dev Dyn. 1995 Feb;202(2):172-80. doi: 10.1002/aja.1002020209.


Molecular mechanisms of endothelial cell differentiation during blood-brain barrier (BBB) development is not well understood due to the lack of specific molecular markers. Here we describe that expression of the mouse multidrug resistance 1a/3 (mdr1a/3) gene can be detected specifically in subsets of vascular endothelial cells associated with neural tissues at as early as embryonic day 10.5 (E10.5). This onset of mdr1a/3 gene expression coincides with the previously described first appearance of morphologically distinct endothelial cells in neural tissues during BBB development. To our knowledge, the mdr1a/3 gene is the earliest endothelial cell differentiation marker gene during BBB development described thus far. In addition, we have found that neither the level nor pattern of mdr1a/3 gene expression in BBB endothelial cells is affected by aberrant cortical neuronal layers in mutant mouse reeler.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis*
  • Animals
  • Blood-Brain Barrier / genetics
  • Brain / blood supply
  • Brain / embryology*
  • Cell Differentiation*
  • Drug Resistance, Multiple / genetics
  • Endothelium, Vascular / embryology*
  • Gene Expression Regulation, Developmental
  • Mice
  • Mice, Mutant Strains
  • Mutation


  • ATP Binding Cassette Transporter, Subfamily B, Member 1