Genes Involved in Sister Chromatid Separation Are Needed for B-type Cyclin Proteolysis in Budding Yeast

Cell. 1995 Apr 21;81(2):269-78. doi: 10.1016/0092-8674(95)90337-2.

Abstract

B-type cyclin destruction is necessary for exit from mitosis and the initiation of a new cell cycle. Through the isolation of mutants, we have identified three essential yeast genes, CDC16, CDC23, and CSE1, which are required for proteolysis of the B-type cyclin CLB2 but not of other unstable proteins. cdc23-1 mutants are defective in both entering and exiting anaphase. Their failure to exit anaphase can be explained by defective cyclin proteolysis. CDC23 is required at the metaphase/anaphase transition to separate sister chromatids, and we speculate that it might promote proteolysis of proteins that hold sister chromatids together. Proteolysis of CLB2 is initiated in early anaphase, but a fraction of CLB2 remains stable until anaphase is complete.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase / genetics
  • Anaphase-Promoting Complex-Cyclosome
  • Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome
  • Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome
  • Blotting, Western
  • Cell Cycle Proteins / genetics
  • Chromatids / genetics*
  • Cyclins / metabolism*
  • Fungal Proteins / genetics*
  • Gene Deletion
  • Genes, Fungal / genetics*
  • Ligases / genetics
  • Mitosis / genetics*
  • Models, Biological
  • Mutagenesis
  • Nuclear Proteins*
  • Nucleocytoplasmic Transport Proteins
  • Precipitin Tests
  • Saccharomyces cerevisiae Proteins*
  • Selection, Genetic
  • Ubiquitin-Conjugating Enzymes*
  • Ubiquitin-Protein Ligase Complexes
  • Yeasts / genetics*

Substances

  • Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome
  • Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome
  • CDC16 protein, S cerevisiae
  • CDC23 protein, S cerevisiae
  • CSE1 protein, S cerevisiae
  • Cell Cycle Proteins
  • Cyclins
  • Fungal Proteins
  • Nuclear Proteins
  • Nucleocytoplasmic Transport Proteins
  • Saccharomyces cerevisiae Proteins
  • Ubiquitin-Conjugating Enzymes
  • Ubiquitin-Protein Ligase Complexes
  • ubiquitin-conjugating enzyme UBC4
  • Anaphase-Promoting Complex-Cyclosome
  • Ligases