Reversions to respiratory competence of omnipotent sup45 suppressor mutants may be caused by secondary sup45 mutations

Curr Genet. 1995 Feb;27(3):195-200. doi: 10.1007/BF00326148.

Abstract

The molecular nature of the sup45 respiratory deficient omnipotent suppressor, and of three reversions to respiratory competence which removed the suppressor effect of the initial mutation, was examined. All reversions were caused by secondary sup45 mutations which indicates a direct connection between sup45 "respiratory" and "translational" functions. Computer analysis showed the local changes of Sup45 protein characteristics in the suppressor strain and revertants in comparison to the wild-type protein. The distribution of mutant sites in relation to evolutionary conserved, and tentatively functional, regions in the Sup45 protein is discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Conserved Sequence
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Genes, Dominant
  • Genes, Fungal*
  • Mitochondria / metabolism
  • Molecular Sequence Data
  • Oxidative Phosphorylation*
  • Peptide Termination Factors*
  • Protein Conformation
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Suppression, Genetic / genetics*

Substances

  • Fungal Proteins
  • Peptide Termination Factors
  • SUP45 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins