Activation of murine epidermal gamma delta T cells through surface 2B4

Eur J Immunol. 1995 Apr;25(4):1117-20. doi: 10.1002/eji.1830250440.


Dendritic epidermal T cells (DETC) are gamma delta T cells that normally reside in murine skin. They express on their surface the 2B4 molecule, a 66-kDa glycoprotein of the immunoglobulin gene superfamily thought to be associated with anti-tumor cytotoxicity by natural killer and lymphokine-activated killer cells. Here, we show that ligation of surface 2B4 transduces cell activation signals in DETC. Treatment with anti-2B4 monoclonal antibodies triggers the secretion of interferon-gamma and interleukin-2 by DETC lines, induces proliferation of resting DETC lines, amplifies anti-CD3-dependent proliferation of DETC freshly isolated from mouse skin; and up-regulates egr-1 and c-fos mRNA expression. These results indicate a unique pathway for DETC activation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Antigens, Surface / immunology
  • Antigens, Surface / metabolism*
  • Cell Line
  • DNA-Binding Proteins / biosynthesis
  • Dendritic Cells / immunology*
  • Early Growth Response Protein 1
  • Epidermis / immunology
  • Female
  • Immediate-Early Proteins*
  • Interferon-gamma / metabolism
  • Interleukin-2 / metabolism
  • Mice
  • Retroviridae Proteins, Oncogenic / biosynthesis
  • Signal Transduction
  • Transcription Factors*
  • Up-Regulation


  • Antibodies, Monoclonal
  • Antigens, Surface
  • DNA-Binding Proteins
  • Early Growth Response Protein 1
  • Egr1 protein, mouse
  • Immediate-Early Proteins
  • Interleukin-2
  • Retroviridae Proteins, Oncogenic
  • Transcription Factors
  • Interferon-gamma