Abstract
Dendritic epidermal T cells (DETC) are gamma delta T cells that normally reside in murine skin. They express on their surface the 2B4 molecule, a 66-kDa glycoprotein of the immunoglobulin gene superfamily thought to be associated with anti-tumor cytotoxicity by natural killer and lymphokine-activated killer cells. Here, we show that ligation of surface 2B4 transduces cell activation signals in DETC. Treatment with anti-2B4 monoclonal antibodies triggers the secretion of interferon-gamma and interleukin-2 by DETC lines, induces proliferation of resting DETC lines, amplifies anti-CD3-dependent proliferation of DETC freshly isolated from mouse skin; and up-regulates egr-1 and c-fos mRNA expression. These results indicate a unique pathway for DETC activation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / pharmacology
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Antigens, Surface / immunology
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Antigens, Surface / metabolism*
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Cell Line
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DNA-Binding Proteins / biosynthesis
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Dendritic Cells / immunology*
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Early Growth Response Protein 1
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Epidermis / immunology
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Female
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Immediate-Early Proteins*
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Interferon-gamma / metabolism
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Interleukin-2 / metabolism
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Mice
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Retroviridae Proteins, Oncogenic / biosynthesis
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Signal Transduction
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Transcription Factors*
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Up-Regulation
Substances
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Antibodies, Monoclonal
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Antigens, Surface
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DNA-Binding Proteins
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Early Growth Response Protein 1
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Egr1 protein, mouse
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Immediate-Early Proteins
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Interleukin-2
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Retroviridae Proteins, Oncogenic
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Transcription Factors
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Interferon-gamma