Detection of 5-HT3R-A, a 5-HT3 receptor subunit, in submucosal and myenteric ganglia of rat small intestine using in situ hybridization

Abstract

Physiological and radioligand binding studies have demonstrated the existence of 5-HT3 receptors in the enteric nervous system. In order to determine if the cloned 5-HT3 receptor subunit, 5-HT3R-A, was expressed in the enteric nervous system of rats, we have performed in situ hybridization with 33P-labeled cRNA antisense probes on sections of rat small intestine. Hybridization was detected in both submucosal and myenteric ganglia of the duodenum, jejunum, and ileum.