Effects of magnesium on platelet aggregation and adhesion. Magnesium modulates surface expression of glycoproteins on platelets in vitro and ex vivo

Thromb Haemost. 1994 Dec;72(6):912-8.


Magnesium deficiency and its association with platelet hyperreactivity has been well recognised in a variety of diseases including myocardial infarction, preeclampsia, and diabetes. In order to investigate potential effects of intravenous Mg2+ supplementation, platelet function was studied by measurements of in vitro bleeding time (BT) and of fibrinogen (Fg)-mediated aggregation of washed platelets. In addition, the effect of Mg2+ on platelet adhesion onto immobilised Fg, on Fg binding to activated platelets, and on surface expression of GMP-140 or GP53 was evaluated. Mg2+ (4 mM) prolonged in vitro BT by 30% and inhibited Fg-mediated aggregation significantly, independent of the agonist used to initiate platelet aggregation (ADP, collagen, epinephrine, thrombin, phorbol ester). Adhesion of resting platelets to immobilised Fg was reduced by 50% in the presence of 2 mM Mg2+. Moreover, Mg2+ reduced Fg binding to ADP- or collagen-stimulated platelets as well as surface expression of GMP-140 with an IC50 of approximately 3 mM. Intravenous administration of Mg2+ to healthy volunteers inhibited both ADP-induced platelet aggregation (p < 0.05) by 40% and binding of Fg or surface expression of GMP-140 by 30% (p < 0.05). Thus, pharmacological concentrations of Mg2+ effectively inhibit platelet function in vitro and ex vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bleeding Time
  • Evaluation Studies as Topic
  • Fibrinogen / metabolism
  • Humans
  • In Vitro Techniques
  • Magnesium / pharmacology*
  • Platelet Adhesiveness / drug effects*
  • Platelet Aggregation Inhibitors / pharmacology*
  • Platelet Membrane Glycoproteins / drug effects*
  • Protein Binding
  • Reference Values


  • Platelet Aggregation Inhibitors
  • Platelet Membrane Glycoproteins
  • Fibrinogen
  • Magnesium