Identification and kinetics of two recently bone marrow-derived B cell populations in peripheral lymphoid tissues

Cell Immunol. 1995 May;162(2):185-93. doi: 10.1006/cimm.1995.1068.


In rats, the glycoprotein Thy-1 is expressed on recently bone marrow (BM)-generated B cells but not on mature recirculating follicular (RF) B cells. Here we demonstrate that Thy-1+ B cells consist of two phenotypically distinct, but developmentally related, populations: a population of newly formed (NF) B cells (IgMbr-IgDdu) that give rise to the second, less immature, Thy-1+ population of so-called early recirculating follicular (ERF) B cells (Thy-1+IgMduIgDbr) cells. These cells ultimately develop to RF-B cells (Thy-1-IgMbrIgDdu). Kinetic studies reveal that in absolute numbers per day most cells die at the transition of NF-B cells in the BM and those in the periphery: less cells die at later stages of B cell differentiation. Given the notion that this cell loss is not random, we speculate that NF-B cells and ERF-B cells may represent crucial steps during peripheral B cell development and their selection. Identification of their unique phenotype makes it possible to evaluate their roles in development of the antibody repertoire.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocyte Subsets / cytology*
  • Bone Marrow Cells*
  • Cell Differentiation
  • Cell Division
  • Female
  • Hematopoiesis
  • Lymphoid Tissue / cytology
  • Male
  • Radiation Chimera
  • Rats
  • Rats, Inbred Strains
  • Thy-1 Antigens / metabolism
  • Time Factors
  • Whole-Body Irradiation


  • Thy-1 Antigens