Cold-shock induction of a family of TIP1-related proteins associated with the membrane in Saccharomyces cerevisiae

Mol Microbiol. 1995 Jan;15(2):341-53. doi: 10.1111/j.1365-2958.1995.tb02248.x.


TIP1 is the first known cold-shock- and heat-shock-induced gene in Saccharomyces cerevislae. Here it is demonstrated that a TIP1 homologue, TIR1, which had been previously cloned as SRP1 (serine-rich protein), is strongly induced by a downshift in growth temperature from 30 to 10 degrees C. We further cloned TIR2, which is transcribed at a low basal level but is increased strongly by cold shock and, to a lesser extent, by heat shock. The predicted protein sequence of TIR2 demonstrates remarkable homology to TIR1 (72.2%) and is also homologous with TIP1 (49%). TIP1, TIR1 and TIR2 are rich in both serine and alanine residues and each contains serine-rich tandem repeats. The proteins contain putative N-terminal signal peptides as well as hydrophobic C-terminal sequences, indicating that the proteins may be membrane bound. The predicted protein sequences are also consistent with extensive O-mannosylation as well as glycosyl-phosphatidyl inositol (GPI) membrane anchoring. Cell fractionation analysis as well as studies using a yeast strain that is conditionally deficient in glycosylation demonstrate that TIP1 is a heavily modified membrane-associated protein. Single, double combinations and triple mutants were created and none demonstrated any obvious phenotype, indicating that this family of genes is not essential for normal growth.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins*
  • Cloning, Molecular
  • Cold Temperature*
  • Fungal Proteins / biosynthesis*
  • Fungal Proteins / genetics
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal*
  • Glycoproteins*
  • Heat-Shock Proteins / biosynthesis*
  • Heat-Shock Proteins / genetics
  • Hot Temperature
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Multigene Family
  • Nuclear Proteins / biosynthesis*
  • Nuclear Proteins / genetics
  • Phenotype
  • Protein Processing, Post-Translational
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • alpha Karyopherins


  • Carrier Proteins
  • Fungal Proteins
  • Glycoproteins
  • Heat-Shock Proteins
  • Membrane Proteins
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • TIP1 protein, S cerevisiae
  • TIR2 protein, S cerevisiae
  • alpha Karyopherins

Associated data

  • GENBANK/L23521