CNS growth factors protect neurons and glia against a wide variety of insults in vitro and in vivo by mechanisms which include buffering toxic rises in intracellular calcium. Cytosolic calcium elevation also plays a key role in complement injury, but the possibility that growth factors protect against antibody-mediated complement attack has not hitherto been addressed. In multiple sclerosis, antibodies and complement appear to contribute to the selective targeting and damage of oligodendrocytes and myelin. Here we have investigated the possibility that growth factors active in oligodendrocyte development and differentiation might protect these cells against injury mediated by antibody and complement in vitro. None was found to be protective.