The use of a double antibody sandwich ELISA and monoclonal antibodies for the assessment of porcine IgM, IgG and IgA concentrations

Vet Immunol Immunopathol. 1995 Feb;44(3-4):309-17. doi: 10.1016/0165-2427(94)05307-e.

Abstract

Double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) have been developed for the assessment of IgM, IgG and IgA concentrations in porcine serum. Isotype-specific monoclonal antibodies (mAb) were used for coating and detection. The DAS-ELISAs were examined for their ability to detect each isotype and for the assay variations. A computer programme was used to verify the parallelism of the slope of each serum sample with the slope of standard/reference serum, a prerequisite for reliable estimation of Ig concentrations. The DAS-ELISAs are easy to perform and highly specific, have adequate detection levels (ranging from 7 to 50 ng ml-1) and are very reproducible, as illustrated by the inter- and intra-assay variation coefficients (ranging between 4.9 and 7%). To illustrate the applicability of the ELISAs we assessed Ig concentrations in pig sera sampled from birth to young adulthood.

MeSH terms

  • Aging / immunology
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Immunoglobulin A / analysis*
  • Immunoglobulin G / analysis*
  • Immunoglobulin M / analysis*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Swine / immunology*

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M