Sequence analysis of HindIII Q2 fragment of capripoxvirus reveals a putative gene encoding a G-protein-coupled chemokine receptor homologue

Virology. 1995 May 10;209(1):207-12. doi: 10.1006/viro.1995.1244.


The DNA sequence of the HindIII Q2 fragment near the left terminus of the capripoxvirus (KS-1 strain) genome was determined. The sequence contains two complete open reading frames (ORFs) and a part of a third. Analysis of the deduced amino acid sequence of one of these ORFs, Q2/3L, revealed that this gene has the capacity to encode a protein which is related to members of the G-protein coupled chemokine receptor subfamily, the swinepoxvirus K2R and the human cytomegalovirus US28 ORFs. It has the key structural characteristics of the G-protein-coupled receptor superfamily, e.g., seven hydrophobic regions, predicted to span the cell membrane, and the cysteine residues in the first and second extracellular loops that are implicated in formation of a disulfide bond. Southern blot analysis showed that all three species of the Capripoxvirus genus, i.e., sheep pox, goat pox, and lumpy skin disease of cattle, contain copies of this putative G-protein-coupled chemokine receptor homologue.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Capripoxvirus / classification
  • Capripoxvirus / genetics*
  • Cattle
  • DNA, Viral / genetics
  • Deoxyribonuclease HindIII
  • GTP-Binding Proteins / metabolism
  • Genes, Viral*
  • Molecular Sequence Data
  • Receptors, Cytokine / genetics
  • Receptors, Cytokine / metabolism
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Viral Proteins / genetics*


  • DNA, Viral
  • Receptors, Cytokine
  • Viral Proteins
  • Deoxyribonuclease HindIII
  • GTP-Binding Proteins

Associated data

  • GENBANK/S78201