Isolation of the human interleukin 10 promoter. Characterization of the promoter activity in Burkitt's lymphoma cell lines

Cytokine. 1995 Jan;7(1):1-7. doi: 10.1006/cyto.1995.1001.


Interleukin 10 (IL-10) is a pleiotropic growth and differentiation factor with potent suppressor functions on macrophages, T cells and NK cells and contributes to the regulation of proliferation and differentiation of B cells. The expression of IL-10 appears to be tightly regulated, as the levels of constitutive expression in normal cells is extremely low. In contrast to normal haematopoetic cells, Epstein-Barr virus (EBV)-immortalized B cells and EBV-positive Burkitt's lymphoma cells express high levels of IL-10 constitutively. In this report we have cloned and sequenced IL-10 promoter fragments and analysed their activity in EBV-positive Burkitt's lymphoma cells. A nested set of DNA fragments from the IL-10 gene 5'-flanking region was placed upstream of the luciferase gene and assayed for their ability to direct luciferase expression in Burkitt's lymphoma cells. We have identified elements within the 5'-flanking region of the human IL-10 gene which can activate or suppress the constitutive expression of IL-10. The essential promoter of the IL-10 gene, which induces low levels of luciferase expression, was found to require the major start site of transcription (+1), a TATA-box (-77) and up to 150 additional 5' nucleotides. Positive regulatory sequences are located between -1100/-900. Negative regulatory elements which abolish luciferase activity were identified between -800/-300.

MeSH terms

  • Base Sequence
  • Burkitt Lymphoma / pathology*
  • Cell Line, Transformed
  • Cell Transformation, Viral
  • Gene Expression Regulation, Neoplastic*
  • Gene Expression Regulation, Viral
  • Genes
  • Genes, Reporter
  • Herpesvirus 4, Human / physiology
  • Humans
  • Interleukin-10 / genetics*
  • Luciferases / biosynthesis
  • Luciferases / genetics
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Recombinant Fusion Proteins / biosynthesis
  • Regulatory Sequences, Nucleic Acid
  • Tumor Cells, Cultured


  • Recombinant Fusion Proteins
  • Interleukin-10
  • Luciferases

Associated data

  • GENBANK/X78437