Cloning and characterization of a chitinase (chit42) cDNA from the mycoparasitic fungus Trichoderma harzianum

Curr Genet. 1994 Dec;27(1):83-9. doi: 10.1007/BF00326583.

Abstract

A cDNA of Trichoderma harzianum (chit42), coding for an endochitinase of 42 kDa, has been cloned using synthetic oligonucleotides corresponding to amino-acid sequences of the purified chitinase. The cDNA codes for a protein of 423 amino acids. Analysis of the N-terminal amino-acid sequence of the chitinase, and comparison with that deduced from the nucleotide sequence, revealed post-translational processing of a putative signal peptide of 22 amino acids and a second peptide of 12 amino acids. The chit42 sequence presents overall similarities with filamentous fungal and bacterial chitinases and to a lesser extent with yeast and plant chitinases. The deduced amino-acid sequence has putative catalytic, phosphorylation and glycosylation domains. Expression of chit42 mRNA is strongly induced by chitin and chitin-containing cell walls and is subjected to catabolite repression. Southern analysis shows that it is present as a single-copy gene in T. harzianum. chit42 is also detected in several tested mycoparasitic and non-mycoparasitic fungal strains.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chitinases / biosynthesis
  • Chitinases / genetics*
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • DNA, Fungal / genetics*
  • Enzyme Induction
  • Fungal Proteins / biosynthesis
  • Fungal Proteins / genetics*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal*
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics*
  • Molecular Sequence Data
  • Protein Conformation
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Trichoderma / enzymology
  • Trichoderma / genetics*

Substances

  • DNA, Complementary
  • DNA, Fungal
  • Fungal Proteins
  • Isoenzymes
  • Chitinases

Associated data

  • GENBANK/S78423