Establishing parasegments in Drosophila embryos: roles of the odd-skipped and naked genes

Dev Biol. 1995 May;169(1):295-308. doi: 10.1006/dbio.1995.1145.

Abstract

During Drosophila embryogenesis, a genetic cascade establishes repeating developmental units, the parasegments, along the anterior-posterior axis. Anterior and posterior boundaries of parasegments are defined by narrow stripes of cells expressing the segment polarity genes engrailed and wingless, respectively. Through single and double mutant analysis, we describe genetic interactions regulating the precise activation of engrailed and wingless in alternate parasegments. The pair-rule gene odd-skipped and the segment polarity gene naked are both required to restrict engrailed expression. odd-skipped represses expression of fushi tarazu, a known activator of engrailed. naked prevents activation of engrailed by fushi tarazu, without affecting fushi tarazu expression. engrailed expression is thus limited to narrow stripes of cells at the anterior boundaries of these parasegments. wingless expression is regulated by both odd-skipped and the pair-rule gene paired. odd-skipped represses wingless expression, while paired restricts the domain of expression of odd-skipped. wingless expression is thus allowed in narrow stripes of cells at the posterior boundaries of these parasegments. Accurate expression of engrailed and wingless is also required for cells within each parasegment to assume their proper positional identity. In odd-skipped mutants, the positional identities of particular cells are changed, creating mirror-image duplications of the body pattern. We present a model describing how the altered expression patterns of fushi tarazu, engrailed, and wingless generate the mutant phenotype.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Drosophila / embryology*
  • Drosophila / genetics
  • Drosophila Proteins*
  • Fushi Tarazu Transcription Factors
  • Gene Expression Regulation
  • Genes, Insect*
  • Genes, Regulator*
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • In Situ Hybridization
  • Insect Hormones / genetics
  • Insect Hormones / metabolism
  • Models, Genetic
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism
  • RNA, Messenger / isolation & purification
  • Receptors, Invertebrate Peptide / genetics
  • Receptors, Invertebrate Peptide / metabolism*
  • Receptors, Neurotransmitter*
  • Receptors, Tachykinin / genetics
  • Receptors, Tachykinin / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Wnt1 Protein

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • En protein, Drosophila
  • Fushi Tarazu Transcription Factors
  • Homeodomain Proteins
  • Insect Hormones
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Receptors, Invertebrate Peptide
  • Receptors, Neurotransmitter
  • Receptors, Tachykinin
  • TkR86C protein, Drosophila
  • Transcription Factors
  • Wnt1 Protein
  • ftz protein, Drosophila
  • prd protein, Drosophila
  • wg protein, Drosophila