HLA-DQA1 typing in Danes by two polymerase chain reaction (PCR) based methods

Forensic Sci Int. 1995 May 9;73(1):1-13. doi: 10.1016/0379-0738(95)01707-0.

Abstract

A total of 280 persons were HLA-DQA1 typed by two different polymerase chain reaction (PCR) based methods; (i) a reverse dot-blot (RDB) method, which can differentiate between six alleles, and (ii) a combined PCR-restriction fragment length polymorphism (PCR-RFLP) and allele-specific amplification (ASA) method, which together recognise eight alleles. In 146 unrelated Danish individuals, the HLA-DQA1 alleles were in Hardy-Weinberg equilibrium. For identity testing, the power of discrimination (PD) of HLA-DQA1 was 0.932 with the RDB method and 0.942 with the PCR-RFLP/ASA method. For paternity testing, the theoretical chance of exclusion in HLA-DQA1 of non-fathers was 0.634 with the RDB method and 0.660 with the PCR-RFLP/ASA method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Base Sequence
  • DNA Primers / genetics
  • Denmark
  • Gene Amplification
  • Gene Frequency
  • HLA-DQ Antigens / genetics*
  • HLA-DQ alpha-Chains
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Restriction Fragment Length
  • Sequence Alignment

Substances

  • DNA Primers
  • HLA-DQ Antigens
  • HLA-DQ alpha-Chains
  • HLA-DQA1 antigen