Abstract
A gene, fdxP, was identified upstream of the rrnA gene in Caulobacter crescentus and shown to encode ferredoxin II (FdII) by insertional inactivation. FdII is homologous to a class of [2Fe-2S] ferredoxins typified by putidaredoxin. Furthermore, reconstitution assays demonstrated that FdII was able to promote p-hydroxybenzoate hydroxylase activity in ferredoxin-depleted extracts. Thus, biodegradation of p-hydroxybenzoate may be ferredoxin dependent in C. crescentus.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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4-Hydroxybenzoate-3-Monooxygenase / metabolism*
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Amino Acid Sequence
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Caulobacter crescentus / enzymology
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Caulobacter crescentus / genetics*
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Caulobacter crescentus / metabolism
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Cell-Free System
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Ferredoxins / classification
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Ferredoxins / genetics*
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Ferredoxins / metabolism
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Molecular Sequence Data
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Mutagenesis, Insertional
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Sequence Homology, Amino Acid
Substances
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Ferredoxins
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4-Hydroxybenzoate-3-Monooxygenase