Risk of cross-colonization and infection by Pseudomonas aeruginosa in a holiday camp for cystic fibrosis patients

J Clin Microbiol. 1995 Mar;33(3):572-5. doi: 10.1128/JCM.33.3.572-575.1995.

Abstract

The risk of cross-colonization and subsequent infection by Pseudomonas aeruginosa in holiday camps for cystic fibrosis patients was studied in 91 children by culturing sputum at their arrival, at their departure, 2 months later, and at regular intervals thereafter. The isolated strains were subjected to serotyping, phage typing, pyocin typing, and genotyping by random amplified polymorphic DNA fingerprinting-PCR. It was concluded from random amplified polymorphic DNA fingerprinting-PCR typing that the Pseudomonas flora was not constant in most children. Some children harbored one genotype, whereas some harbored two or more different genotypes simultaneously. Most culture-positive children easily acquired a strain of another genotype which replaced the former one or coexisted with the original one. The incidence of sputum conversion was 7.7% in previously negative children; the incidence of permanent colonization and infection was 1.9%. This risk was comparable with that observed in the community. We conclude that the risk of cross-infection is trivial compared with the obvious joy and social benefit derived from a holiday camp.

MeSH terms

  • Adolescent
  • Bacteriophage Typing
  • Base Sequence
  • Camping
  • Child
  • Child, Preschool
  • Community-Acquired Infections / genetics
  • Community-Acquired Infections / microbiology*
  • Cystic Fibrosis / microbiology*
  • DNA Fingerprinting
  • DNA, Bacterial / analysis
  • Female
  • Genotype
  • Humans
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Pseudomonas Infections / microbiology*
  • Pseudomonas aeruginosa / classification
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / isolation & purification*
  • Pyocins / analysis
  • Serotyping

Substances

  • DNA, Bacterial
  • Pyocins