Region II of the Haemophilus influenzae type be capsulation locus is involved in serotype-specific polysaccharide synthesis

Mol Microbiol. 1995 Jan;15(1):107-18. doi: 10.1111/j.1365-2958.1995.tb02225.x.

Abstract

The central (serotype-specific) Region II of the Haemophilus influenzae Type b capsulation locus cap is 8.3 kb long and contains a cluster of four genes. We show that these genes, designated orf1 to orf4, are involved in the biosynthetic steps required for the formation of the Type b capsular polysaccharide and that orf1 probably encodes a CDP-ribitolpyrophosphorylase. We present evidence that growth of polysaccharide chains takes place through the alternating addition of single sugar nucleotides.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Capsules / biosynthesis
  • Bacterial Capsules / genetics*
  • Base Sequence
  • Blotting, Northern
  • Chromosome Mapping
  • DNA Probes / genetics
  • Gene Expression / genetics
  • Genes, Bacterial*
  • Haemophilus influenzae / classification
  • Haemophilus influenzae / genetics*
  • Haemophilus influenzae / metabolism
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Nucleoside Diphosphate Sugars / metabolism
  • Nucleotidyltransferases / genetics*
  • Nucleotidyltransferases / metabolism
  • Open Reading Frames / genetics
  • Serotyping

Substances

  • DNA Probes
  • Nucleoside Diphosphate Sugars
  • cytidine diphosphate ribitol
  • Nucleotidyltransferases
  • D-ribitol-5-phosphate cytidylyltransferase

Associated data

  • GENBANK/X78559