Molecular characterization of a gene that confers 2-deoxyglucose resistance in yeast

Yeast. 1994 Sep;10(9):1195-202. doi: 10.1002/yea.320100907.

Abstract

We have isolated a gene whose expression enables yeast cells to overcome the inhibition of growth produced by the presence of 2-deoxyglucose. The gene contains an open reading frame of 738 bp that may code for a protein of 27,100 Da. Cells carrying this gene contain high levels of a specific 2-deoxyglucose-6-phosphate phosphatase. The expression of this phosphatase is increased by the presence of 2-deoxyglucose and is constant along the growth curve.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Chromosome Mapping
  • Chromosomes, Fungal
  • Deoxyglucose / pharmacology*
  • Drug Resistance / genetics*
  • Genes, Fungal*
  • Molecular Sequence Data
  • Phenotype
  • Phosphoric Monoester Hydrolases / chemistry*
  • Phosphoric Monoester Hydrolases / genetics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid

Substances

  • Saccharomyces cerevisiae Proteins
  • Deoxyglucose
  • DOG2 protein, S cerevisiae
  • Phosphoric Monoester Hydrolases

Associated data

  • GENBANK/U03107