bcl-2 is expressed in neurons that survive focal ischemia in the rat

Neuroreport. 1995 Jan 26;6(2):394-8. doi: 10.1097/00001756-199501000-00040.


Expression of the proto-oncogene bcl-2 prevents programmed cell death in vitro, but it is not known whether bcl-2 plays a role in determining cell survival after cerebral ischemia. Using immunohistochemistry and Western blot analysis, bcl-2 protein expression was studied in the rat brain 24 h following 60 or 120 min of temporary focal ischemia. Sixty minutes of ischemia induced bcl-2 protein in neurons throughout the frontoparietal cortex in non-infarcted regions, whereas 120 min of ischemia induced bcl-2 in neurons only just outside the margin of the infarction. bcl-2 protein was also induced in glial cells, mainly microglia, border zone of the infarction. In the infarcted regions of caudate and cortex, bcl-2 protein was exclusively induced in endothelial cells and the vessel walls. Western blot revealed a characteristic single band at 26 kDa only in ischemic samples. These data show that bcl-2 is induced in sublethally injured cells and suggest that bcl-2 could play a role in determining cell survival in cerebral ischemia.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apoptosis / genetics
  • Blotting, Western
  • Cell Survival
  • Disease Models, Animal
  • Gene Expression
  • Immunohistochemistry
  • Ischemic Attack, Transient / genetics*
  • Male
  • Neurons / pathology*
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-bcl-2
  • Rats
  • Rats, Sprague-Dawley


  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2