High-resolution mapping of the physical location of the tomato Cf-2 gene

Mol Plant Microbe Interact. Mar-Apr 1995;8(2):200-6. doi: 10.1094/mpmi-8-0200.

Abstract

To isolate the tomato Cf-2 resistance gene by map-based cloning, plants recombinant for RFLP markers close to Cf-2 were selected by exploiting the flanking morphological markers yv (yellow virescent) and tl (thiaminless). Using these recombinants, a high-resolution linkage map of the region encompassing the Cf-2 gene has been generated containing several new RFLP markers. Mapping of two YAC clones carrying Lycopersicon esculentum and L. peruvianum DNA, indicates that in both genotypes the physical distance between the two closet flanking markers is less than 40 kb. This study also positions Cf-2 relative to the Mi gene, which confers resistance to root-knot nematodes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Mapping*
  • Chromosomes, Artificial, Yeast
  • Cosmids
  • DNA Primers
  • Genes, Plant*
  • Lycopersicon esculentum / genetics*
  • Molecular Sequence Data
  • Plant Proteins / genetics
  • Polymorphism, Restriction Fragment Length

Substances

  • DNA Primers
  • Plant Proteins