Determination of saffron (Crocus sativus L.) components in crude plant extract using high-performance liquid chromatography-UV-visible photodiode-array detection-mass spectrometry

J Chromatogr A. 1995 May 5;699(1-2):107-18. doi: 10.1016/0021-9673(95)00044-n.

Abstract

The determination of saffron components in crude plant extracts by high-performance liquid chromatography-UV-visible photodiode-array detection on-line with mass spectrometry is described. The method is shown to be suitable for the determination of picrocrocin, the glycosidic precursor of safranal, safranal and flavonoids; it is the technique of choice for the analysis of crocetin glycosides (crocins) carrying one up to five glucoses and differentiation of their trans and cis isomers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Carotenoids / analysis
  • Chromatography, High Pressure Liquid / methods*
  • Cyclohexenes
  • Flavonoids / analysis
  • Glucosides / analysis
  • Mass Spectrometry / methods*
  • Molecular Sequence Data
  • Plant Extracts / chemistry*
  • Spectrophotometry, Ultraviolet
  • Spices / analysis*
  • Terpenes / analysis

Substances

  • Cyclohexenes
  • Flavonoids
  • Glucosides
  • Plant Extracts
  • Terpenes
  • Carotenoids
  • crocin
  • picrocrocin