Suppression of type IV collagenase in MDA-MB-435 human breast cancer cells by eicosapentaenoic acid in vitro and in vivo

Cancer Lett. 1995 May 25;92(1):21-6. doi: 10.1016/0304-3835(95)03752-i.


Dietary supplementation with 4% eicosapentaenoic acid (EPA), a long-chain polyunsaturated fatty acid, suppressed the development of lung metastases in nude mice from MDA-MB-435 human breast cancer cell mammary fat pad solid tumors. Zymography of primary tumor homogenates showed that this inhibition of metastasis was associated with reduced levels of 92-kDa type IV collagenase gelatinolytic activity; this isoform has been previously associated with the metastatic phenotype. The 72-kDa isoform, which was also present, may have arisen from the cancer cells, or tumor-associated host cells. In vitro, the incorporation of EPA, 0.25-1.0 micrograms/ml into the medium caused a concentration-related suppression of cultured MDA-MB-435 cell 92-kDa type IV collagenase mRNA expression.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Northern
  • Breast Neoplasms / enzymology*
  • Breast Neoplasms / pathology
  • Carcinoma, Ductal, Breast / enzymology*
  • Carcinoma, Ductal, Breast / pathology
  • Collagenases / biosynthesis
  • Eicosapentaenoic Acid / pharmacology*
  • Female
  • Humans
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase Inhibitors*
  • Mice
  • Mice, Nude
  • Neoplasm Metastasis
  • Neoplasm Transplantation
  • RNA, Messenger / analysis
  • RNA, Neoplasm / analysis
  • Tumor Cells, Cultured


  • Matrix Metalloproteinase Inhibitors
  • RNA, Messenger
  • RNA, Neoplasm
  • Eicosapentaenoic Acid
  • Collagenases
  • Matrix Metalloproteinase 9