The antiproliferative effect of vitamin D analogs on MCF-7 human breast cancer cells

Cancer Lett. 1995 May 25;92(1):77-82. doi: 10.1016/0304-3835(95)03765-o.

Abstract

We analyzed the antiproliferative effect of 1,25-dihydroxyvitamin D3 and four vitamin D analogs on MCF-7, a human breast cancer cell line known to express the vitamin D receptor. Growth curve studies and [3H]thymidine incorporation assays were used to assess the antiproliferative effect of 1,25-dihydroxyvitamin D3 (vitamin D), Ro 23-7553, Ro 24-5531, Ro 25-5317, and Ro 24-5583. Growth of MCF-7 cells was significantly inhibited by 1,25-dihydroxyvitamin D3 and all four analogs at 10(-8) M (P < 0.05). MCF-7 cells treated with analog had significantly less [3H]thymidine incorporation than cells treated with 1,25-dihydroxyvitamin D3 (P < 0.05). The affinity of the analogs for the vitamin D receptor was similar to that of 1,25-dihydroxyvitamin D3. These results demonstrate that analogs of 1,25-dihydroxyvitamin D3 are potent antiproliferative agents on human breast cancer cells and that this activity is likely mediated through the vitamin D receptor.

MeSH terms

  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology*
  • Binding, Competitive
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Calcitriol / analogs & derivatives*
  • Calcitriol / metabolism
  • Calcitriol / pharmacology*
  • Cell Division / drug effects
  • Humans
  • Receptors, Calcitriol / metabolism
  • Tumor Cells, Cultured

Substances

  • Receptors, Calcitriol
  • Ro 24-5531
  • Calcitriol
  • Ro 23-7553