Different requirements for NSF, SNAP, and Rab proteins in apical and basolateral transport in MDCK cells

Cell. 1995 May 19;81(4):571-80. doi: 10.1016/0092-8674(95)90078-0.

Abstract

We used an in vitro system based on streptolysin O-permeabilized MDCK cells to study the involvement of NSF, SNAP, SNAREs, and Rab proteins in polarized membrane transport of epithelial cells. In MDCK cells, transport from the trans-Golgi network (TGN) to the basolateral plasma membrane is inhibited by anti-NSF antibodies and stimulated by alpha-SNAP. In contrast, transport from the TGN to the apical cell surface is not affected by anti-NSF antibodies or alpha-SNAP. Furthermore, apical transport is insensitive to Rab-GDI and tetanus and botulinum neurotoxins, which inhibit basolateral transport. These results provide evidence that the Rab-NSF-SNAP-SNARE mechanism operates in basolateral transport, while other molecules constitute the machinery for vesicular delivery in the apical pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Carrier Proteins / metabolism*
  • Cell Polarity
  • Cells, Cultured
  • Dogs
  • Epithelium / metabolism
  • GTP-Binding Proteins / metabolism*
  • Kidney / metabolism
  • Membrane Proteins / metabolism*
  • N-Ethylmaleimide-Sensitive Proteins
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Vesicular Transport Proteins*

Substances

  • Carrier Proteins
  • Membrane Proteins
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Vesicular Transport Proteins
  • GTP-Binding Proteins
  • N-Ethylmaleimide-Sensitive Proteins