Structural characterization, chromosomal localization and phylogenetic evaluation of two pairs of AGAMOUS-like MADS-box genes from maize

Gene. 1995 Apr 24;156(2):155-66. doi: 10.1016/0378-1119(95)00020-7.


In order to gain a better understanding of the development and evolution of cereal flowers, we have cloned and sequenced two MADS-box genes from maize and the partial cDNA of a third one. One of the genomic clones was identified as ZAG2 (Zea AGAMOUS 2), while the other has a very similar structure and the potential to encode a protein which shares 94% sequence identity with the putative ZAG2 gene product. The cDNA reveals considerable similarity to ZAG1. Phylogenetic evaluation of the sequence information, as well as chromosomal localization, both suggest that we have identified two pairs of AGAMOUS-like MADS-box genes which were created during duplication of chromosomal segments or complete chromosomes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • AGAMOUS Protein, Arabidopsis
  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • Genes, Plant / genetics*
  • Genome
  • MADS Domain Proteins
  • Molecular Sequence Data
  • Multigene Family
  • Phylogeny
  • Plant Proteins / genetics*
  • Protein Structure, Secondary
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Transcription Factors / genetics*
  • Zea mays / genetics*


  • AGAMOUS Protein, Arabidopsis
  • DNA-Binding Proteins
  • MADS Domain Proteins
  • Plant Proteins
  • Transcription Factors
  • ZAG1 protein, Zea mays
  • ZAG2 protein, Zea mays

Associated data

  • GENBANK/X80206
  • GENBANK/X81199
  • GENBANK/X81200