Beware of mycoplasmas

Trends Biotechnol. 1993 Apr;11(4):143-51. doi: 10.1016/0167-7799(93)90089-R.


Mycoplasma infection of cell cultures is widespread and has major detrimental effects on cellular physiology and metabolism. Since cell culture is used extensively, both in research and in industrial production processes, questions of primary concern arise, such as: how can mycoplasma contamination be detected; what are the effects of such contamination on cellular functions; what methods are available for eliminating contamination?

Publication types

  • Review

MeSH terms

  • Animals
  • Cells, Cultured / microbiology*
  • Cells, Cultured / ultrastructure
  • Humans
  • Microscopy, Electron
  • Mycoplasma / isolation & purification*
  • Mycoplasma / ultrastructure